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Green fluorescent protein

properties, applications, and protocols
  • 385 Pages
  • 0.14 MB
  • English

Wiley-Liss , New York
Green fluorescent pr
Other titlesGFP, green fluorescent protein
Statementedited by Martin Chalfie, Steven Kain.
ContributionsChalfie, Martin., Kain, Steven.
LC ClassificationsQP552.G73 G47 1998
The Physical Object
Paginationxiii, 385 p. :
ID Numbers
Open LibraryOL690982M
ISBN 10047117839X
LC Control Number97038102

Green Fluorescent Protein: Properties, Applications, and Protocols, Second Edition is essential reading for researchers and graduate students in molecular and cellular biology, physiology, biochemistry, developmental biology, neuroscience, ecology, in 4/5(1).

Green fluorescent protein (GFP) is a fluorescent protein that was originally isolated from the luminous organ of the jellyfish Aequorea victoria by Dr.

Osamu Shimomura Green fluorescent protein. Some organisms have evolved fluorescent molecules (perhaps used to attract prey) that, like the toxins listed above, have been harnessed for. [13] Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization in Bacillus subtilis Chris D.

Webb, Orna Resnekov Pages Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences.

With this tool, it is now possible to visualize Green fluorescent protein book any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant 5/5(1). Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences.

With this tool, it is now possible to visualize nearly any. Green Fluorescent Protein: Properties, Applications and Protocols, Edition 2 - Ebook written by Martin Chalfie, Steven R.

Kain. Read this book using Google Play Books app on your PC, android, iOS devices. Download for offline reading, highlight, bookmark or take notes while you read Green Fluorescent Protein: Properties, Applications and Protocols, Edition 2. Green Fluorescent Protein. Editors: Hicks, Barry W. (Ed.) Free Preview.

Buy this book eBook ,69 € price for Spain (gross) Buy eBook ISBN ; Digitally watermarked, DRM-free; Included format: PDF, EPUB; ebooks can be used on all reading devices; Immediate eBook download after purchase. Introduction. Although the green fluorescent protein (GFP) has a scientific history predatingI like to credit the Febru issue of the journal Science (Fig.

1) as the beginning of the GFP cover image, 1 showing green-glowing sensory neurons in C. elegans, was instantly recognized as representing a major breakthrough for cell by:   Since the discovery of green fluorescent protein, chemists have found different ways to change its properties.

For example, a different amino acid in this region makes the protein glow with blue color. Chemists have also performed more complex modifications, like this one, that makes the protein emit the red color.

Thomas’s cowardly behavior is never examined deeply enough. Smith is more on target with “Green Fluorescent Protein,” in which Green fluorescent protein book Max fights his attraction to another male teenager.

Max’s mother, a recovering alcoholic, gets her own story, (“Funny Weird or Funny Ha Ha?), a meandering lament for her dead husband. Monitoring of Physiological Processes:S. Kupzig, S. San Lee and G. Banting, Membrane Trafficking.C.

Description Green fluorescent protein FB2

Kaether and H.-H. Gerdes, Monitoring of Protein Secretion with Green Fluorescent Protein.R.M. Hoffman, Green Fluorescent Protein to Visualize Cancer Progression and Metastasis.X.

Zhao, T. Duong, C.-C. Huang, S.R. Kain and X. Li, Comparison of. In Aequorea victoria a protein called aequorin releases blue light upon binding with calcium.

This blue light is then totally absorbed by the GFP, which in turn gives off the green light as in the animation below. In GFP was cloned. Now GFP is found in laboratories all over the world where it is used in every conceivable plant and animal. GREEN FLUORESCENT PROTEIN monomer molecular weight.

Shimomura (6) proteolyzed denatured GFP, ana-lyzed the peptide that retained visible absorbance, and correctly proposed that the chromophore is a 4-(p-hydroxybenzylidene)imidazolidinone attached to the peptide backbone through the 1- and 2-positions of the ring. Douglas C.

Prasher (born August ) is an American molecular is known for his work to clone and sequence the genes for the photoprotein aequorin and green fluorescent protein (GFP) and for his proposal to use GFP as a tracer molecule. He communicated his pioneering work to Martin Chalfie and Roger Y.

Tsien, but by was himself unable to Alma mater: Ohio State University. Page 16 Learn more: Green Fluorescent Protein (GFP) GFP is a tiny protein from jellyfish that glows when exposed to ultraviolet Size: KB. Green Fluorescent Protein: Properties, Applications, and Protocols is the first book devoted to this rapidly evolving reporter system.

This collection of essays from pioneers in the field tackles both theory and practice, offering numerous case studies, examples, illustrations, and troubleshooting tips. The book clearly demonstrates how to tailor GFP to specific systems, maximize.

Expression and Purification of recombinant Green Fluorescent Protein (rGFP) from E. coli using Ni2+-Agarose Column Chromatography. Andrea Bustamante Janakikeerthika Darmarpandi Abstract Green Fluorescent Proteins are vital components of bioluminescence in marine animals. Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from diverse marine animals are widely used as universal genetically encoded fluorescent labels.

Many laboratories have focused their efforts on identification and development of fluorescent proteins with novel characteristics and enhanced properties, resulting in a powerful toolkit for Cited by: Purchase Green Fluorescent Protein, Volume - 1st Edition.

Print Book & E-Book. ISBNGeneral description This book demonstrates the power and the versatility of the green fluorescent protein (GFP) marker with a cutting-edge collection of readily reproducible GFP techniques. Biochemical and physical properties of green fluorescent protein / William W.

Ward: The three-dimensional structure of green fluorescent protein and its implications for function and design / George N. Phillips, Jr: Molecular biology and mutation of green fluorescent protein / David A.

Zacharias, Roger Y. TsienPages:   The first three stories were my favourites- 'Isolettes', 'Green Fluorescent Protein', and 'The B9ers'. I got this book in a bookstore that was closing down for less than $ As far as I'm concerned it was a fantastic deal that introduced me to an author that I hope releases more short stories soon/5.

Green Fluorescent Protein: Properties, Applications and Protocols, Vol.

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47, 2nd Edition (Methods of Biochemical Analysis) (Volume 47) by Chalfie, M. & Kain, S.R. and a great selection of related books, art and collectibles available now at Deals with the utility of green fluorescent protein (GFP).

This volume presents describes techniques in a context that allows comparisons to other related methodologies - such comparisons are valuable to readers who must adapt existing procedures to new systems.

Green Fluorescent Protein Applications in Drosophila (Tulle Hazelrigg and Jennifer H. Mansfield). The Uses of Green Fluorescent Protein in Plants (Jim Haseloff and Kirby R. Siemering). Uses of GFP in Transgenic Vertebrates (Sean Magason, Adam Amsterdam, Nancy Hopkins, and Shuo Lin).

Foremost among the fluorescent proteins used for these purposes by cell biologists is the green fluorescent protein, isolated (like aequorin) from the jellyfish Aequoria victoria. This protein is encoded in the normal way by a single gene that can be Author: Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter.

Green fluorescent protein (GFP) is a protein that occurs naturally in the jellyfish Aequorea victoria. The purified protein appears yellow under ordinary lighting but glows bright green under sunlight or ultraviolet light. The protein absorbs energetic blue and ultraviolet light and emits it as a lower energy green light via fluorescence.

The. “This book focuses on a very important area of application of green fluorescent protein (GFP) and its variants. The significance of viral applications of fluorescent proteins is well covered.

it will certainly be a valuable source of information and a good basis for laboratory protocols for researchers from all different fields of Brand: Humana Press. Book Title. eLS. Additional Information. How to Cite.

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Shaner, N. Green Fluorescent Protein (GFP). eLS. Author Information. The Scintillon Institute, San Diego, California, USA. Based in part on the previous version of this eLS article ‘Green Fluorescent Protein (GFP)’ () by Leila D Hebshi, Brigitte M Angres, Xianqiang L Li.

In one study, Phomopsis viticola was transformed by GFP (green fluorescent protein) using protoplast mediated transformation and penetration and. Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis.

The original was isolated from Discosoma, and named are now available that fluoresce orange, red, and far-red.Advances in fluorescent proteins, live-cell imaging, and superresolution instrumentation have ushered in a new era of investigations in cell biology, medicine, and physiology.

From the identification of the green fluorescent protein in the jellyfish Aequorea victoria to .Cubitt was a founding scientist at Aurora, where he was responsible for improving green fluorescent protein technology and providing the technical support for the development of Aurora's intellectual property and licensing strategy for fluorescent proteins.